Plasmid

Part:BBa_K2908671:Design

Designed by: Dong Yufan   Group: iGEM19_CSU_CHINA   (2019-10-16)

- We use the pLN431 as the back-clone of the Module1, then we cut the vector and an insert sequence (lacZp+lacZ) with MluI and NotI to form the pocket plasmid.
T--CSU_CHINA--lacZ.png
- We designed the BsmBI restriction digest location on the 5’ and 3’ top of the inserted sequence, the following figure shows the pattern of construction method.
T--CSU_CHINA--ggmodule1.png